Molecular epidemiology of tuberculosis in Prague: Analysis by restriction fragment length polymorphism
نویسندگان
چکیده
منابع مشابه
Restriction fragment length polymorphism (RFLP) analysis and tuberculosis epidemiology.
In order to study polymorphisms of the DNA insertion sequence 6110 (IS6110) in Mycobacterium tuberculosis strains isolated from Colombian patients, together with resistance to antituberculous medications in the Department of Quindío, Colombia, a prospective study was conducted using a consecutive sample of 59 patients with symptomatic pulmonary tuberculosis whose cases had been confirmed by bac...
متن کامل[Molecular epidemiology of tuberculosis by the use of IS6110 restriction fragment length polymorphism: a study from 2001 to 2003].
OBJECTIVE To analyze the situation of tuberculosis infection by DNA fingerprinting in the middle and eastern part of Osaka, Japan. DESIGN We performed IS6110 restriction fragment length polymorphism (RFLP) on 1200 isolates from tuberculosis patients who visited our hospital from January 2001 to December 2003. A cluster was defined as a series of isolates with more than 90% similarity by IS611...
متن کاملRestriction fragment length polymorphism typing of Mycobacterium tuberculosis.
Restriction fragment length polymorphism (RFLP) typing of Mycobacterium tuberculosis is a recently developed technique which allows strains of tuberculosis to be differentiated with a high degree of confidence. The application of this technique has enabled the patterns of transmission of tuberculosis to be elucidated with far greater certainty than was previously possible. This review will disc...
متن کاملRestriction Fragment Length Polymorphism (RFLP)
Restriction Fragment Length Polymorphism (RFLP) is a technique in which organisms may be differentiated by analysis of patterns derived from cleavage of their DNA. If two organisms differ in the distance between sites of cleavage of a particular restriction endonuclease, the length of the fragments produced will differ when the DNA is digested with a restriction enzyme. The similarity of the pa...
متن کاملDirect early identification of Mycobacterium tuberculosis by PCR-restriction fragment length polymorphism analysis from clinical samples.
We attempted to apply the PCR-restriction fragment length polymorphism (PCR-RFLP) technique for the early detection and identification of Mycobacterium tuberculosis directly from clinical samples. PCR-RFLP of hsp65 was applied to the DNA extracted directly from sputum samples (n = 226) collected from 226 patients. We could detect and identify M. tuberculosis in 84.5% of the acid-fast bacillus (...
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ژورنال
عنوان ژورنال: International Journal of Infectious Diseases
سال: 1998
ISSN: 1201-9712
DOI: 10.1016/s1201-9712(98)90119-9